Date of Award
12-17-2005
Document Type
Thesis
Abstract
To elucidate the roles of key neurochemicals in the metabolic suppression of Arctic ground squirrels, a technique is required that is capable of on-line, in vivo, high temporal resolution, quantitative detection of neurotransmitters. A capillary electrophoresis instrument has been built that is coupled to microdialysis by flow-gated injection. Primary amines recovered in vivo via microdialysis are derivatized on-column by cyanide-catalyzed formation of fluorescent isoindoles with naphthalene-2,3-dialdehyde for laser-induced fluorescence detection. The system is capable of detecting base-line glutamate in vivo in 15-second intervals.
Recommended Citation
Swearingen, Kristian Edward, "Development and characterization of a capillary electrophoresis instrument with laser-induced fluorescence detection for on-line monitoring of glutamate in vivo via microdialysis" (2005). Chemistry and Biochemistry. 46.
https://ualaska.researchcommons.org/uaf_grad_chem_biochem/46
Handle
http://hdl.handle.net/11122/6112